8KDX

Tau-S214 Phosphorylation Inhibits Fyn Kinase Interaction and Increases the Decay Time of NMDAR-mediated Current

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	31.152, 35.558, 73.169
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.002 - 1.010
Rwork	0.173
R-free	0.18540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	2.157
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.002	1.030
High resolution limit [Å]	1.010	1.010
Number of reflections	43333	2839
<I/σ(I)>	32.1
Completeness [%]	99.6
Redundancy	9.6
CC(1/2)	0.990	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	1.2 M Sodium citrate tribasic dehydrated, 0.1 M Tris buffer pH8.5