8KDK
Crystal structure of CcbF in complex with PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2021-11-16 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.391, 102.700, 102.954 |
| Unit cell angles | 90.00, 92.44, 90.00 |
Refinement procedure
| Resolution | 45.990 - 1.800 |
| R-factor | 0.2037 |
| Rwork | 0.203 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.976 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.990 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.108 | 0.794 |
| Number of reflections | 144884 | 7101 |
| <I/σ(I)> | 11.2 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.2 |
| CC(1/2) | 0.998 | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tris-Cl pH 8.5, 31% PEG 4000, 0.16M NaOAc |
