8KD8
N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF N-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 62.200, 110.670, 69.474 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.438 - 2.581 |
Rwork | 0.173 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 2.192 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.9) |
Phasing software | MrBUMP (2.2.6) |
Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.438 | 2.700 |
High resolution limit [Å] | 2.580 | 2.580 |
Number of reflections | 7832 | 927 |
<I/σ(I)> | 4.4 | 4.4 |
Completeness [%] | 99.8 | 99.2 |
Redundancy | 12.2 | 12.3 |
CC(1/2) | 0.998 | 0.942 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 293 | 0.2M potassium sodium tartrate tetrahydrate, 20% w/v polyethylene glycol 3350, cryoprotected with 40% Morpheus precipitant mix 3 |