8KCL

Crystal Structure of M- and C-Domains of the shaft pilin LrpA from Ligilactobacillus ruminis - Triclinic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98030
Spacegroup name	P 1
Unit cell lengths	36.334, 47.677, 51.720
Unit cell angles	107.25, 101.52, 111.78

Refinement procedure

Resolution	39.460 - 1.470
R-factor	0.17545
Rwork	0.175
R-free	0.19106
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.460	1.491
High resolution limit [Å]	1.465	1.465
Rmerge	0.110	0.444
Rmeas	0.130	0.545
Rpim	0.069	0.308
Number of reflections	47229	2307
<I/σ(I)>	6.9	2.1
Completeness [%]	95.2	92.4
Redundancy	3.4	3
CC(1/2)	0.987	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M Lithium Sulphate, 0.1 M Tris pH 8.5, 30% PEG 4000