8KCE

Structure of RvY_06210 at 1.05 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	C 1 2 1
Unit cell lengths	89.362, 64.020, 45.987
Unit cell angles	90.00, 109.54, 90.00

Refinement procedure

Resolution	37.020 - 1.050
R-factor	0.1299
Rwork	0.129
R-free	0.15450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.893
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.020	1.070
High resolution limit [Å]	1.050	1.050
Rmerge	0.039	0.576
Rmeas	0.046	0.676
Rpim	0.025	0.352
Number of reflections	108442	5112
<I/σ(I)>	14.9	1.8
Completeness [%]	95.8
Redundancy	3.4
CC(1/2)	0.998	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Magnesium chloride hexahydrate, 0.03M Tris-HCl pH8.2 , 19% w/v PEG 4000