8KB9
Structure of the WDR91 WD40 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.721, 154.353, 92.314 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.330 - 1.900 |
| R-factor | 0.2136 |
| Rwork | 0.212 |
| R-free | 0.23840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.545 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.330 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.055 | 1.081 |
| Number of reflections | 28408 | 1807 |
| <I/σ(I)> | 21.6 | |
| Completeness [%] | 99.7 | |
| Redundancy | 13.4 | |
| CC(1/2) | 0.999 | 0.794 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M ammonium citrate tribasic pH 7.0, 8% PEG 3350 |
