8KB2
Crystal Structure of M- and C-Domains of the shaft pilin LrpA from Ligilactobacillus ruminis - iodide derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.54980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.903, 50.898, 125.623 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.580 - 1.910 |
| R-factor | 0.1811 |
| Rwork | 0.179 |
| R-free | 0.21149 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.433 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.580 | 1.945 |
| High resolution limit [Å] | 1.905 | 1.905 |
| Rmerge | 0.129 | 0.970 |
| Rmeas | 0.130 | |
| Rpim | 0.018 | 0.141 |
| Number of reflections | 21167 | 1058 |
| <I/σ(I)> | 24.2 | 4.8 |
| Completeness [%] | 97.6 | 85.5 |
| Redundancy | 48.4 | 45.5 |
| CC(1/2) | 0.999 | 0.969 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1 M sodium acetate pH 4.6, 25% PEG 4000 |
