8KAQ

Glycoside hydrolase family 1 beta-glucosidase (E318G mutant) from Streptomyces griseus (sophorose complex)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	91.793, 100.179, 96.048
Unit cell angles	90.00, 102.87, 90.00

Refinement procedure

Resolution	46.824 - 2.130
Rwork	0.183
R-free	0.22330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.372
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.824	2.170
High resolution limit [Å]	2.130	2.130
Number of reflections	93387	4558
<I/σ(I)>	10.4
Completeness [%]	98.7
Redundancy	3.4
CC(1/2)	0.995	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Bis-Tris propane (pH 6.5), 0.3 M sodium formate, 18% PEG3350