8KAP
Glycoside hydrolase family 1 beta-glucosidase from Streptomyces griseus (ligand-free)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-07 |
Detector | DECTRIS PILATUS3 S 2M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 96.858, 97.756, 184.874 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.261 - 2.200 |
Rwork | 0.232 |
R-free | 0.25680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.863 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.430 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 89749 | 4548 |
<I/σ(I)> | 8.8 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.6 | |
CC(1/2) | 0.991 | 0.685 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris (pH 5.5), 0.2 M ammonium acetate, 15% (w/v) PEG3350 |