8KAO

Glutamate dehydrogenase-69O

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	99.782, 153.599, 110.550
Unit cell angles	90.00, 115.58, 90.00

Refinement procedure

Resolution	45.040 - 2.550
R-factor	0.21054
Rwork	0.208
R-free	0.26022
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.321
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.040	2.630
High resolution limit [Å]	2.550	2.550
Rmerge	0.166	1.164
Rmeas	0.180	1.259
Rpim	0.068	0.476
Total number of observations		30810
Number of reflections	48864	4486
<I/σ(I)>	10.3	1.8
Completeness [%]	100.0
Redundancy	6.9	6.9
CC(1/2)	0.995	0.708

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Potassium phosphate monobasic/Sodium phosphate dibasic buffer (pH 6.2), 10% (w/v) polyethylene glycol (PEG) 3,000, and 10% (v/v) glycerol.