8KAI
Crystal structure of SpyCas9-crRNA-tracrRNA complex bound to 17nt target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 144.300, 130.186, 146.413 |
| Unit cell angles | 90.00, 104.02, 90.00 |
Refinement procedure
| Resolution | 48.570 - 3.490 |
| R-factor | 0.2282 |
| Rwork | 0.225 |
| R-free | 0.29390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.779 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.460 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rpim | 0.126 | 0.514 |
| Number of reflections | 73038 | 3650 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1.0 M Sodium chloride, 0.1 M Sodium citrate pH 6.0, 13% PEG4000 |
