8KAG
Crystal structure of SpyCas9 in complex with sgRNA and target RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-07-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 185.681, 185.681, 137.162 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.920 - 3.880 |
R-factor | 0.3036 |
Rwork | 0.303 |
R-free | 0.32020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.019 |
Data reduction software | HKL-3000 (7.21) |
Data scaling software | HKL-3000 (7.21) |
Phasing software | PHENIX (1.17.1_360) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.950 |
High resolution limit [Å] | 3.880 | 3.880 |
Rpim | 0.100 | 0.374 |
Number of reflections | 27876 | 1267 |
<I/σ(I)> | 5.33 | |
Completeness [%] | 99.8 | 99.3 |
Redundancy | 6.9 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1.7 M Ammonium citrate tribasic, 0.1 M HEPES pH 7.0 |