8KA2

Crystal structure of the RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) from Vibrio vulnificus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9795
Spacegroup name	P 6
Unit cell lengths	207.726, 207.726, 54.647
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.420 - 3.380
R-factor	0.28789
Rwork	0.286
R-free	0.32212
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.312
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.440
High resolution limit [Å]	3.380	3.380
Number of reflections	17817	721
<I/σ(I)>	11.1
Completeness [%]	99.6
Redundancy	6.2
CC(1/2)	0.996	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	22% (w/v) PEG 3350 and 4% (v/v) Tacsimate (pH 6.0)