8K8M
Crystallographic structure of Lysozyme C from chicken
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-21 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54056 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.537, 78.537, 37.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.000 - 1.600 |
| R-factor | 0.16051 |
| Rwork | 0.159 |
| R-free | 0.18789 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.803 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.062 | 0.583 |
| Rmeas | 0.066 | 0.642 |
| Rpim | 0.022 | 0.265 |
| Total number of observations | 4510 | |
| Number of reflections | 16043 | 784 |
| <I/σ(I)> | 21.2 | 3 |
| Completeness [%] | 99.8 | |
| Redundancy | 8.3 | 5.8 |
| CC(1/2) | 0.999 | 0.808 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 4.6 | 298 | 5%(w/v) NaCl, 0.1M Na acetate pH4.6 |
