8K7X

Crystal structure of GH146 beta-L-arabinofuranosidase Bll3HypBA1 (amino acids 380-1223) in complex with Tris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-14
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	59.592, 111.571, 153.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.760 - 1.750
R-factor	0.14414
Rwork	0.143
R-free	0.16730
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.640
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.760	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.176	1.014
Rpim	0.102	0.592
Number of reflections	103620	5038
<I/σ(I)>	8.9
Completeness [%]	100.0	100
Redundancy	6.7	6.8
CC(1/2)	0.993	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	12% PEG 8000, 0.2 M MgCl2, and 0.1 M Tris-HCl (pH 8.5)