8K7N
Structure of ferric-binding protein KfuA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.275, 52.278, 56.392 |
Unit cell angles | 90.00, 94.43, 90.00 |
Refinement procedure
Resolution | 37.580 - 2.000 |
R-factor | 0.1625 |
Rwork | 0.158 |
R-free | 0.19880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.864 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.290 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rpim | 0.031 | 0.073 |
Number of reflections | 18079 | 882 |
<I/σ(I)> | 20.6 | |
Completeness [%] | 96.7 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M Sodium chloride, 25% w/v Polyethylene glycol 3,350 and 0.1 M Tris |