8K70
Structural basis for the distinct roles of non-conserved Pro116 and conserved Tyr124 of BCH domain of yeast p50RhoGAP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 61 |
Unit cell lengths | 108.110, 108.110, 250.803 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.810 - 2.810 |
R-factor | 0.2125 |
Rwork | 0.211 |
R-free | 0.23950 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.212 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.640 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.230 | |
Number of reflections | 37961 | 2084 |
<I/σ(I)> | 19.7 | |
Completeness [%] | 93.8 | |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.1 M Bis-Tris propane pH 7.0 and 2.1 M NaCl |