8K6T

The minor pilin structure of FctB3 in Streptococcus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-01
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.100
Spacegroup name	P 43 2 2
Unit cell lengths	44.335, 44.335, 181.092
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.060 - 2.800
R-factor	0.2114
Rwork	0.209
R-free	0.25960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.540
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.060	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	4687	2261
<I/σ(I)>	10.7
Completeness [%]	96.2
Redundancy	3.2
CC(1/2)	0.995	0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	ammonium sulfate, sodium acetate trihydrate