8K07
Pseudouridine 5'-monophosphate glycosylase from Arabidopsis thaliana -- citrate bound K185A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-04-09 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 57.299, 68.518, 73.095 |
Unit cell angles | 117.88, 94.31, 109.38 |
Refinement procedure
Resolution | 24.796 - 1.121 |
R-factor | 0.2053 |
Rwork | 0.205 |
R-free | 0.21920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.153 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.160 |
High resolution limit [Å] | 1.120 | 2.410 | 1.120 |
Rmerge | 0.051 | 0.037 | 0.624 |
Rmeas | 0.072 | 0.053 | 0.882 |
Rpim | 0.051 | 0.037 | 0.624 |
Total number of observations | 611922 | ||
Number of reflections | 316141 | 31850 | 28136 |
<I/σ(I)> | 6.5 | ||
Completeness [%] | 92.4 | 93 | 82.1 |
Redundancy | 1.9 | 1.9 | 1.9 |
CC(1/2) | 0.993 | 0.992 | 0.590 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1M Sodium citrate pH6.5, 16% PEG 10000 |