8K05
Pseudouridine 5'-monophosphate glycosylase from Arabidopsis thaliana -- sulfate bound holoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-04-09 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 3 |
Unit cell lengths | 121.126, 121.126, 121.126 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.550 - 1.450 |
R-factor | 0.2058 |
Rwork | 0.206 |
R-free | 0.21710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.059 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.500 |
High resolution limit [Å] | 1.450 | 3.120 | 1.450 |
Rmerge | 0.111 | 0.054 | 3.706 |
Rmeas | 0.113 | 0.055 | 3.794 |
Rpim | 0.024 | 0.012 | 0.810 |
Total number of observations | 2288355 | ||
Number of reflections | 104528 | 10687 | 10315 |
<I/σ(I)> | 6 | ||
Completeness [%] | 99.9 | 99.2 | 100 |
Redundancy | 21.9 | 20.6 | 21.9 |
CC(1/2) | 0.999 | 0.999 | 0.455 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1M Cacodylate pH6.5, 1.4M Ammonium sulfate |