8JZK
Crystal structure of glutathione S-transferase Tau7 from Salix lindleyana in complex with GSH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2023-03-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979183 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.727, 87.931, 113.973 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.340 - 2.550 |
R-factor | 0.19172701895 |
Rwork | 0.188 |
R-free | 0.25835 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.146 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Refinement software | PHENIX (1.9_1692+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.340 | 2.550 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmeas | 0.233 | |
Rpim | 0.071 | |
Number of reflections | 10186 | 724 |
<I/σ(I)> | 9.7 | |
Completeness [%] | 99.3 | |
Redundancy | 5.6 | |
CC(1/2) | 0.978 | 0.978 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.1M BIS-TRIS propane pH 9.0, 8% w/v Polyethylene glycol 20000 |