8JYQ
Crystal structure of cancer-specific anti-HER2 antibody H2Mab-214 in complex with epitope peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.143, 62.793, 78.122 |
| Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
| Resolution | 36.460 - 1.750 |
| R-factor | 0.1977 |
| Rwork | 0.196 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.881 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.580 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 70833 | 11237 |
| <I/σ(I)> | 10.95 | 1.05 |
| Completeness [%] | 98.6 | 97.2 |
| Redundancy | 3.86 | 3.83 |
| CC(1/2) | 0.998 | 0.708 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M CHES pH 9.5, 20% w/v PEG 8000 |
