8JYB

Crystal Structure of Intracellular B30.2 Domain of VpBTN3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	BRUKER METALJET
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-02
Detector	Bruker PHOTON III
Wavelength(s)	1.34138
Spacegroup name	P 21 21 21
Unit cell lengths	43.996, 68.056, 134.657
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.050 - 1.910
R-factor	0.1601
Rwork	0.158
R-free	0.20090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zxk
RMSD bond length	0.011
RMSD bond angle	1.659
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.050	1.940
High resolution limit [Å]	1.910	1.910
Rmerge	0.035	0.115
Number of reflections	32076	1394
<I/σ(I)>	31.08
Completeness [%]	99.4
Redundancy	6.12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M Bis Tris pH 5.5, 2.0 M ammonium sulfate