8JVX

Crystal structure of the dimeric DZBB fold protein mkaL2_v1.2_Z

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-23
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	34.463, 37.705, 52.239
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.573 - 1.801
R-factor	0.2048
Rwork	0.203
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.470
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.800	1.800
Number of reflections	12049	1919
<I/σ(I)>	10.51
Completeness [%]	99.1
Redundancy	3.5
CC(1/2)	0.991	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% 2-propanol, 100mM Tris-HCl pH8.5, 30% PEG 3350