8JVW

Crystal structure of the dimeric DZBB fold protein tkoL2_v1.2_Z

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	I 2 2 2
Unit cell lengths	23.959, 44.427, 84.289
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.145 - 1.806
R-factor	0.2128
Rwork	0.211
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.024
RMSD bond angle	1.855
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	7929	1257
<I/σ(I)>	18.77
Completeness [%]	99.9
Redundancy	3.4
CC(1/2)	0.990	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	70% MPD, 100mM HEPES pH7.0