8JVT
Crystal structure of the chimeric protein mkaL2_v1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-07-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.137, 53.887, 55.654 |
| Unit cell angles | 90.00, 113.41, 90.00 |
Refinement procedure
| Resolution | 38.684 - 2.106 |
| R-factor | 0.2069 |
| Rwork | 0.202 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.267 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 35872 | 5832 |
| <I/σ(I)> | 0.78 | |
| Completeness [%] | 93.3 | |
| Redundancy | 1.82 | |
| CC(1/2) | 0.990 | 0.440 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 6000, 100mM Tris pH8.0, 200mM Lithium chrolide |
