8JVR

Crystal structure of the dimeric RIFT fold protein Ph1_GG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	34.449, 34.449, 55.085
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.834 - 1.794
R-factor	0.2388
Rwork	0.238
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.698
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.794	1.794
Number of reflections	6734	2934
<I/σ(I)>	12.5
Completeness [%]	98.2
Redundancy	2.9
CC(1/2)	0.980	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50% PEG400, 100mM Sodium acetate/Acetic acid pH4.5, 200mM Lithium sulfate