8JUF

Crystal structure of human MMP-7 in complex with inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00000
Spacegroup name	I 4
Unit cell lengths	77.220, 77.220, 62.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.600 - 1.390
R-factor	0.19786
Rwork	0.197
R-free	0.22006
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.028
RMSD bond angle	2.431
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.600	1.470
High resolution limit [Å]	1.390	1.390
Number of reflections	37250	5755
<I/σ(I)>	19.77	0.42
Completeness [%]	100.0	100
Redundancy	19.9	18.8
CC(1/2)	1.000	0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	10% w/v Polyethylene glycol 8000, 100mM Tris pH7.0, 200mM Magnesium chloride