8JT0
Dihydrofolate reductase-like enzyme from Leptospira interrogans with additional NADP+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-08-02 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 85.232, 138.280, 236.568 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.960 - 2.200 |
R-factor | 0.2079 |
Rwork | 0.206 |
R-free | 0.24700 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.116 |
Data reduction software | XDS (VERSION Mar 15, 2019 BUILT=20191211) |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHENIX (1.14_3260) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.960 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.084 | 0.880 |
Rmeas | 0.090 | 0.944 |
Rpim | 0.033 | 0.341 |
Number of reflections | 142065 | 6985 |
<I/σ(I)> | 13.7 | 2.4 |
Completeness [%] | 99.8 | |
Redundancy | 7.5 | |
CC(1/2) | 0.997 | 0.776 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 100 mM sodium acetate, 200 mM ammonium sulfate, 25-27% PEG 550 MME |