8JSZ
Crystal structure of a uridylate cyclase from Anabaena sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 07A |
| Synchrotron site | NSRRC |
| Beamline | TPS 07A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-21 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.661, 50.792, 71.391 |
| Unit cell angles | 71.21, 83.96, 62.07 |
Refinement procedure
| Resolution | 25.050 - 1.830 |
| R-factor | 0.17765 |
| Rwork | 0.177 |
| R-free | 0.17893 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.731 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.910 |
| High resolution limit [Å] | 1.830 | 1.840 |
| Number of reflections | 47377 | 4637 |
| <I/σ(I)> | 27.5 | |
| Completeness [%] | 97.6 | 95.1 |
| Redundancy | 5.5 | 5.3 |
| CC(1/2) | 0.998 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M BIS-TRIS pH 6.5, 0.2 M Magnesium chloride hexahydrate, 25% w/v Polyethylene glycol 3,350 |
