8JSC

Structure of the FSP1 protein from Human

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	65.820, 39.126, 70.345
Unit cell angles	90.00, 108.95, 90.00

Refinement procedure

Resolution	39.500 - 2.160
R-factor	0.1884
Rwork	0.187
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.282
Data reduction software	XDS
Data scaling software	ADDREF
Phasing software	PHENIX (v1.13)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.250	2.270
High resolution limit [Å]	2.160	2.160
Number of reflections	18472	2689
<I/σ(I)>	4.1
Completeness [%]	99.4
Redundancy	3.6
CC(1/2)	0.972	0.556

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	PEG 3350, Ammonium nitrate.