8JRB
Structure of DNA polymerase 1 from Aquifex pyrophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.969 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.826, 74.725, 129.560 |
| Unit cell angles | 90.00, 108.25, 90.00 |
Refinement procedure
| Resolution | 46.260 - 2.200 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.23290 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.261 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.910 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rpim | 0.031 | 0.505 |
| Number of reflections | 80316 | 4294 |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 97.5 | 96.8 |
| Redundancy | 4.7 | 4.9 |
| CC(1/2) | 0.994 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 18% PEG 8K, 100 mM Ammonium Sulphate |
