8JR4

Molecular structure of bat MHC II at 2.4 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 42 21 2
Unit cell lengths	92.160, 92.160, 107.365
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.430 - 2.300
R-factor	0.21457
Rwork	0.212
R-free	0.26309
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.542
Data reduction software	HKL-2000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.140	2.140
Rmerge	0.189	1.073
Number of reflections	22821	2453
<I/σ(I)>	15.667
Completeness [%]	100.0
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2 M Calcium acetate hydrate,20% w/v Polyethylene glycol 3,350,pH7.5