8JQ3

Crystal structure of L-rhamnose isomerase from Lactobacillus rhamnosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-19
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	89.700, 139.940, 147.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.510 - 1.900
R-factor	0.16264
Rwork	0.160
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.183
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.086	0.028	0.689
Rmeas	0.089	0.029	0.715
Number of reflections	146232	1822	10740
<I/σ(I)>	22.94
Completeness [%]	100.0
Redundancy	13.5
CC(1/2)	0.999	1.000	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	PEG 550MME, MES