8JOZ

Crystal structure of CmoM from E. coli complexed with sinefungin and cellularly expressed tRNA Ser

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9795
Spacegroup name	P 61 2 2
Unit cell lengths	65.614, 65.614, 584.532
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.740 - 2.220
R-factor	0.1999
Rwork	0.199
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.955
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.740	2.300
High resolution limit [Å]	2.220	2.220
Number of reflections	38928	3750
<I/σ(I)>	16.92
Completeness [%]	99.9
Redundancy	35.6
CC(1/2)	0.999	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.04 M Magnesium acetate tetrahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.0, 30% v/v (+/-)-2-Methyl-2,4-pentanediol