8JOY
Plk1 polo-box domain bound to HPV4 L2 residues 251-257 with pThr255
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2023-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.106, 54.691, 57.578 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 39.650 - 2.610 |
| R-factor | 0.2034 |
| Rwork | 0.198 |
| R-free | 0.25460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.179 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.112 | 0.503 |
| Number of reflections | 6436 | 315 |
| <I/σ(I)> | 5.19 | |
| Completeness [%] | 95.5 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M malate MES Tris buffer pH 6.5, 25% polyethylene glycol 1500 |
