8JOK

Crystal structure of V30L mutated human transthyretin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-24
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	43.160, 85.280, 64.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.510 - 1.640
R-factor	0.1908
Rwork	0.189
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.873
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.350	1.730
High resolution limit [Å]	1.640	1.640
Rmerge	0.093	0.634
Number of reflections	29774	4265
<I/σ(I)>	10.7	2.4
Completeness [%]	99.9
Redundancy	6.5
CC(1/2)	0.997	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	293	PEG400, MgCl2, glycerol