8JOI

Crystal Structure of Bel-1 E166A Mutant.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-11
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5418
Spacegroup name	P 6
Unit cell lengths	123.498, 123.498, 70.644
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.613 - 1.700
Rwork	0.161
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.992
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.350	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.130
Rmeas	0.140
Rpim	0.050
Number of reflections	67521	3585
<I/σ(I)>	12.2
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)	0.998	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M imidazole, 0.1M MES monohydrate, 20% ETG, 10% PEG 8000, 0.1M Sodium formate, 0.1M Ammonium acetate, 0.1M Sodium citrate tribasic dihydrate, 0.1M Potassium sodium tartrate, 0.1M Sodium oxamate