8JNX
alkaline amylase Amy703 with truncated of N-terminus domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-25 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97890 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 171.307, 171.307, 167.170 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.458 - 3.203 |
R-factor | 0.209187704736 |
Rwork | 0.207 |
R-free | 0.24457 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.483 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.079 | |
Number of reflections | 24396 | 1955 |
<I/σ(I)> | 35 | |
Completeness [%] | 98.1 | |
Redundancy | 13.7 | |
CC(1/2) | 0.779 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.1 M Tris-HCl, pH 7.5, 2.4 M Sodium malonate dibasic |