8JNT
RxLR effector RxLR19 from Phytophthora capsici
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 |
| Unit cell lengths | 49.741, 49.741, 61.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.077 - 2.201 |
| R-factor | 0.175 |
| Rwork | 0.174 |
| R-free | 0.20300 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.058 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 43.077 |
| High resolution limit [Å] | 2.200 | 2.201 |
| Rmerge | 0.157 | 0.901 |
| Number of reflections | 4403 | 4403 |
| <I/σ(I)> | 17.75 | |
| Completeness [%] | 100.0 | |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.03M Citric acid, 0.07M BIS-TRIS propane pH 7.6, 20% w/v PEG 3350 |
