8JL5

Crystal structure of the Green fluorescent protein SEA227D variant at pH 4.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	298
Detector technology	CCD
Collection date	2022-06-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	73.935, 82.947, 180.132
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.470 - 1.800
R-factor	0.186
Rwork	0.185
R-free	0.22180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.032
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.140	0.086	0.461
Rmeas	0.146	0.090	0.491
Rpim	0.041	0.024	0.165
Total number of observations	1136537
Number of reflections	102810	10814	10037
<I/σ(I)>	4.3
Completeness [%]	99.2	99.6	98.3
Redundancy	11.1	13.3	8
CC(1/2)	0.992	0.997	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M Citric acid pH 4.5, 20% (w/v) PEG 6000