8JKS

T95R mutant IRF4 DNA-binding domain bound to an DNA containing GAGA motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-13
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9752
Spacegroup name	P 21 21 21
Unit cell lengths	52.610, 68.010, 246.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.020 - 3.300
R-factor	0.2376
Rwork	0.235
R-free	0.28690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8jkl
RMSD bond length	0.003
RMSD bond angle	0.987
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.030	3.390
High resolution limit [Å]	3.300	3.300
Rmerge	0.251	1.382
Rmeas	0.262	1.437
Rpim	0.072	0.388
Total number of observations	180176	13384
Number of reflections	13978	981
<I/σ(I)>	10.1	2.8
Completeness [%]	99.7
Redundancy	12.9	13.6
CC(1/2)	0.995	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M sodium citrate (pH 5.0) and 10% (w/v) PEG 6000