8JKN

T95R mutant IRF4 DNA-binding domain bound to an DNA containing GAAA motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-13
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9752
Spacegroup name	P 31 2 1
Unit cell lengths	118.642, 118.642, 154.647
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.750 - 2.920
R-factor	0.2212
Rwork	0.220
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jm4
RMSD bond length	0.006
RMSD bond angle	1.197
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.750	3.070
High resolution limit [Å]	2.920	2.920
Rmerge	0.261	1.769
Rmeas	0.270	1.826
Rpim	0.068	0.448
Total number of observations	432182	64448
Number of reflections	27994	4003
<I/σ(I)>	12.2	2.6
Completeness [%]	100.0
Redundancy	15.4	16.1
CC(1/2)	0.994	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M MgCl2, 0.1 M sodium citrate (pH 5.0) and 15% (w/v) PEG 4000