8JI9
Crystal Structure of cas7 and AcrIE10 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-25 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.770, 46.240, 133.500 |
| Unit cell angles | 90.00, 101.29, 90.00 |
Refinement procedure
| Resolution | 43.640 - 2.540 |
| R-factor | 0.2389 |
| Rwork | 0.238 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.254 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.640 | 2.610 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.122 | |
| Rmeas | 0.133 | |
| Rpim | 0.052 | |
| Number of reflections | 30500 | 15719 |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.7 | |
| CC(1/2) | 0.997 | 0.699 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 300 | 4% v/v TacsimateTM pH 7.0, 12% w/v Polyethylene glycol 3,350 |
