8JHW

The first crystal structure of a H-2Kb-restricted decapeptide from Cryptosporidium parvum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2021-11-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 65 2 2
Unit cell lengths	86.940, 86.940, 293.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.359 - 3.120
Rwork	0.245
R-free	0.28790
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.380	3.340
High resolution limit [Å]	3.120	3.120
Number of reflections	12577	2207
<I/σ(I)>	15
Completeness [%]	100.0
Redundancy	15.93
CC(1/2)	0.999	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20%(w/v) Polyethylene glycol 3350; 0.2M Lithium acetate dihydrate