8JHV
The first crystal structure of a H-2Kb-restricted decapeptide from Cryptosporidium parvum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-11-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97915 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 89.380, 89.380, 296.370 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.105 - 3.470 |
Rwork | 0.230 |
R-free | 0.29678 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.160 | 3.800 |
High resolution limit [Å] | 3.470 | 3.470 |
Number of reflections | 18018 | 4242 |
<I/σ(I)> | 11.3 | |
Completeness [%] | 99.9 | |
Redundancy | 20 | |
CC(1/2) | 0.999 | 0.875 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20%(w/v) Polyethylene glycol 3350; 0.2M Lithium acetate dihydrate |