8JHL
GDP-bound KRAS G12D in complex with YK-8S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-01 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 82.353, 82.353, 97.912 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.176 - 2.100 |
| R-factor | 0.169452761471 |
| Rwork | 0.165 |
| R-free | 0.20774 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.058 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.180 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 21385 | 12586 |
| <I/σ(I)> | 25.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 17.8 | |
| CC(1/2) | 1.000 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 200mM calcium acetate,100mM MES(PH 5.5-6.5),PEG 8000 16%-24% |
