8JG4

Crystal Structure of YAF9A YEATS bound to H3K27cr peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	110
Detector technology	PIXEL
Collection date	2020-07-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	66.331, 117.700, 59.053
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.110 - 2.300
R-factor	0.1747
Rwork	0.172
R-free	0.21180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.910
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.132	0.052	0.897
Rmeas	0.139	0.055	0.953
Rpim	0.043	0.017	0.314
Total number of observations	216550
Number of reflections	21078	1192	1038
<I/σ(I)>	5
Completeness [%]	99.0	99.8	98.6
Redundancy	10.3	9.4	8.8
CC(1/2)	0.992	0.998	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Tris pH 8.5, 0.2 M Li2SO4, 25% PEG 3350