8JFU
AcrIIA15 in complex with palindromic DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97851 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.604, 131.388, 234.183 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.130 - 3.150 |
| R-factor | 0.2599 |
| Rwork | 0.259 |
| R-free | 0.27480 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.111 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.220 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 30070 | 1942 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.5 | |
| CC(1/2) | 0.999 | 0.604 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Sodium chloride, 0.1 M HEPES-NaOH pH 7.5, 25% w/v PEG 3,350 |
