8JFR

N-terminal domain of AcrIIA15 in complex with palindromic DNA substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97851
Spacegroup name	P 43
Unit cell lengths	58.712, 58.712, 240.593
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.660 - 3.100
R-factor	0.2468
Rwork	0.246
R-free	0.25890
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.293
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.150
High resolution limit [Å]	3.100	3.100
Number of reflections	14903	725
<I/σ(I)>	24.9
Completeness [%]	99.9
Redundancy	4.7
CC(1/2)	1.000	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	1.6 M sodium citrate pH 6.05